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Anti-SSB TRUEMAB Antibody Clone OTI1E11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500406 anti-SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) 100ul $325 In Stock
LC401091 SSB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500406 Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11) 100ug $450 3-4 weeks
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WB(3)
IP(2)
IF(2)
FC(1)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human SSB expression vector
Clone NameClone OTI1E11 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration2.8 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 46.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB), transcript variant 1
Alternative NameLa; La/SSB; LARP3
Database LinkNP_003133
Entrez Gene 6741 Human
Entrez Gene 20823 Mouse
Entrez Gene 81783 Rat
Entrez Gene 710344 Monkey
Entrez Gene 478787 Dog
FunctionLa is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus
Related PathwayStem cell - PluripotencyTranscription Factors Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB(RC205013).
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Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).
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Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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Figure from citation: Immunoprecipitation of SSB(La) protein level by using anti-SSB antibody in HeLa cells. View Citation
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Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500406),(Red), compared to a nonspecific negative control antibody,(Blue).
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