OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody All anti-SSB antibodies

Anti-SSB TRUEMAB Antibody Clone OTI1E11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
  • anti-SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401091) , 20ug Explanation
100ul $325 In Stock
  • Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human SSB expression vector
Clone NameClone OTI1E11 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration2.8 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 46.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB), transcript variant 1
Alternative NameLa; La/SSB; LARP3
Database LinkNP_003133
Entrez Gene 6741 Human
Entrez Gene 20823 Mouse
Entrez Gene 81783 Rat
Entrez Gene 478787 Dog
FunctionLa is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus
Related PathwayStem cell - PluripotencyTranscription Factors Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB(RC205013).
Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).
Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Figure from citation: Immunoprecipitation of SSB(La) protein level by using anti-SSB antibody in HeLa cells. View Citation
Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500406),(Red), compared to a nonspecific negative control antibody,(Blue).


Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title