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Anti-CHEK2 TRUEMAB Antibody Clone OTI2F4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CHEK2 (NM_007194)
|Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|FC, WB, IF
||WB 1:500~1000, IF 1:50~100, FLOW 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
Entrez Gene 11200 Human
Entrez Gene 114212 Rat
Entrez Gene 713668 Monkey
Entrez Gene 486338 Dog
|In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition,this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.|
|Protein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway|
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HEK293T cells transfected with either RC201278 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CHEK2 antibody(TA500405), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
Anti-CHEK2 mouse monoclonal antibody (TA500405) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500405).
Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-CHEK2 monoclonal antibody. (1:200)
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CHEK2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).