Anti-MAP2K4 Antibody, clone 8A8

Also for MAP2K4 (NM_003010)

Gene Name	Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4)
Synonyms:	JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1
Immunogen	Protein expressed in 293T cell transfected with human MAP2K4 expression vector
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 8A8	Isotype	IgG2b
Species Reactivity	Human , Dog	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Background	This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver
Related Pathway	Apoptosis MAPK signaling pathway Toll-like receptor signaling pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (TA500403)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MAP2K4 mouse monoclonal antibody. (TA500403)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MAP2K4 mouse monoclonal antibody. (TA500403)

IHC Image

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (TA500403)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (TA500403)

IF Image

Anti-MAP2K4 mouse monoclonal antibody (TA500403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4(RC206051).

IF Image

Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (TA500403).

IP Image

Immunoprecipitation(IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC206051 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAP2K4 antibody(TA500403), and then analyzed by flow cytometry.

OriGene Antibodies in recent publications

Anti-MAP2K4 TrueMAB Antibody clone 8A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens