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Home Antibody All anti-PLK1 antibodies

Anti-PLK1 TRUEMAB Antibody clone 8C12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500393
  • anti-PLK1 mouse monoclonal antibody,clone 8C12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417592) , 20ug Explanation
100ul 379 In Stock
CF500393
  • Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody,clone 8C12
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IP(1)
IF(1)
FC(1)
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Also for PLK1 (NM_005030)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human PLK1 expression vector
Clone Nameclone 8C12 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration0.96 mg/ml
Guaranteed Application *WB, IF, IP, FC Suggested DilutionsWB 1:1000~2000, IF 1:100, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 68.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
Entrez Gene 5347 Human
Entrez Gene 697686 Monkey
Entrez Gene 489971 Dog
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly,the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.
IF Image
Anti-PLK1 mouse monoclonal antibody (TA500393) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1(RC201795).
IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC201795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLK1 antibody(TA500393), and then analyzed by flow cytometry.

 

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