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Home Antibody All anti-PLK1 antibodies

Anti-PLK1 TRUEMAB Antibody clone 3F12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500383
  • anti-PLK1 mouse monoclonal antibody,clone 3F12
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417592) , 20ug
100ul 325 In Stock
CF500383
  • Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody,clone 3F12
100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(1)
FC(1)
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Also for PLK1 (NM_005030)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human PLK1 expression vector
Clone Nameclone 3F12 IsotypeIgG1
Species ReactivityHuman Concentration1.01 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Related Pathway
Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.</title><style>.an6q{position:absolute;clip:rect(487px,auto,auto,487px);}</style><div class=an6q>According to your current <a href=http://paydayloansforsure.com >need cash now</a> you really look at.</div>
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500383)</title><style>.an6q{position:absolute;clip:rect(487px,auto,auto,487px);}</style><div class=an6q>According to your current <a href=http://paydayloansforsure.com >need cash now</a> you really look at.</div>
IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.</title><style>.an6q{position:absolute;clip:rect(487px,auto,auto,487px);}</style><div class=an6q>According to your current <a href=http://paydayloansforsure.com >need cash now</a> you really look at.</div>
FC Image
HEK293T cells transfected with either RC201795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLK1 antibody(TA500383), and then analyzed by flow cytometry.</title><style>.an6q{position:absolute;clip:rect(487px,auto,auto,487px);}</style><div class=an6q>According to your current <a href=http://paydayloansforsure.com >need cash now</a> you really look at.</div>

 

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