Anti-TRPM4 Antibody, clone 10H5

Also for TRPM4 (NM_017636)

OriGene Data

Immunogen	Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
Clone Name	clone 10H5	Isotype	IgG1
Species Reactivity	Human , Dog , Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB 1:5000~10000, IHC 1:50,
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1
Alternative Name	PFHB1B; TRPM4B
Database Link	NP_060106
Function	Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (RC216888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-TRPM4 mouse monoclonal antibody. (TA500381)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (TA500381)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (TA500381)

IHC Image

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-TRPM4 mouse monoclonal antibody. (TA500381)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TRPM4 mouse monoclonal antibody. (TA500381)

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OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-TRPM4 TrueMAB Antibody clone 10H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data