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AK1 (Locus ID 203) Antibody

TrueMABTM Antibodies - Made against Authentic Protein Antigens
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Catalog No. Description Amount Price Shipping *
TA500321
  • Purified AK1 mouse monoclonal antibody,clone 19D1
100 ul $348 Immediate
  • HEK293T cell transient overexpression lysate as a positive control.(LY400171)
20 ug Included for FREE!
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Also for AK1 (NM_000476)
Gene NameHomo sapiens adenylate kinase 1 (AK1) Synonyms:
ImmunogenProtein expressed in 293T cell transfected with human AK1 expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 19D1 IsotypeIgG2b
Species ReactivityHuman Concentration1.10 mg/ml
Purification Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *WB, IF Suggested DilutionsWB 1:2000
BackgroundAdenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.
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Western blot analysis of 35ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-AK1 antibody.
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Western blot analysis of 35ug of cell extracts from human (HeLa) cells using anti-AK1 antibody.
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Western blot analysis of 35ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-AK1 antibody.
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Western blot analysis of 35ug of cell extracts from Green monkey Kiney (COS7) cells using anti-AK1 antibody.
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Western blot analysis of 35ug of cell extracts from human brain tissue lysates using anti-AK1 antibody.
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Immunofluorescent staining of HT29 cells using anti-AK1 mouse monoclonal antibody (TA500321).

 

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