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Home Antibody All anti-NEUROG1 antibodies

Anti-NEUROG1 TRUEMAB Antibody Clone OTI3F9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500305 NEUROG1 (Neurogenin 1) mouse monoclonal antibody, clone OTI3F9 (formerly 3F9) 100ul $379 In Stock
LC401855 NEUROG1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500305 Carrier-free (BSA/glycerol-free) NEUROG1 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9) 100ug $450 4 Weeks
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WB(2)
IP(1)
IHC(8)
IF(1)
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Also for NEUROG1 (NM_006161)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NEUROG1 (NP_006152) produced in HEK293T cell.
Clone NameClone OTI3F9 IsotypeIgG1
Species ReactivityHuman Concentration1.00mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:500~1000, IHC 1:50, IF 1:100, IP 2-4ug/ml
Predicted MW Explanation 25.7 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target Nameneurogenin 1
Alternative NameAKA|Math4C|NEUROD3|bHLHa6|ngn1
Database LinkNP_006152
Entrez Gene 4762 Human
Function
Related PathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG1 (RC207029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG1.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NEUROG1 monoclonal antibody.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NEUROG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500305)
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IF Image
Anti-NEUROG1 mouse monoclonal antibody (TA500305) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEUROG1(RC207029).
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IP Image
Immunoprecipitation(IP) of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibodies (Negative control: IP without adding anti-NEUROG1 antibody.). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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