Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *
WB, IF
Suggested Dilutions
WB 1:500~1000, IF 1:50~100,
Background
AFP is a major plasma protein produced by the yolk sac and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin.
Related Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
