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Home Antibody All anti-KRT8 antibodies
KO-Validated

Anti-KRT8 TRUEMAB Antibody Clone OTI4D12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500216 CK8 (Keratin 8) mouse monoclonal antibody, clone OTI4D12 (formerly 4D12) 100ul $325 In Stock
LC419425 KRT8 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500216 Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12) 100ug $450 4 Weeks
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WB(3)
IHC(1)
IF(2)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Clone NameClone OTI4D12 IsotypeIgG2a
Species ReactivityHuman , Dog , Monkey Concentration1.00mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~1000, IHC 1:50, IF 1:100,
Predicted MW Explanation 53.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens keratin 8 (KRT8), transcript variant 2
Alternative NameCARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Database LinkNP_002264
Entrez Gene 3856 Human
Entrez Gene 705390 Monkey
Entrez Gene 486513 Dog
FunctionKeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# LC810123) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody TA500216,(1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500216)
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IF Image
Anti-KRT8 mouse monoclonal antibody (TA500216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
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IF Image
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500216).
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