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Home Antibody All anti-AKT3 antibodies

Anti-AKT3 TRUEMAB Antibody clone 9H8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500080
  • anti-AKT3 mouse monoclonal antibody,clone 9H8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401675) , 20ug Explanation
100ul 325 In Stock
CF500080
  • Carrier-free (BSA/glycerol-free) AKT3 mouse monoclonal antibody,clone 9H8
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(1)
IF(2)
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Also for AKT3 (NM_005465)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3
Clone Nameclone 9H8 IsotypeIgG3
Species ReactivityHuman, Mouse Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500, IHC 1;50, IF (1:100)
Predicted MW Explanation 55.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 3 (AKT3), transcript variant 1
Alternative NameMPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Database LinkNP_005456
Entrez Gene 10000 Human
Entrez Gene 23797 Mouse
FunctionAKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1)
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3(RC221051) (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-AKT3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (TA500080) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
IF Image
Anti-AKT3 mouse monoclonal antibody (TA500080) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3(RC221051)
IF Image
Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (TA500080).

 

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