AKT3 (Gene ID 10000) Antibody

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Catalog No.	Description	Amount	Price	Shipping *
TA500080	Purified anti-AKT3 mouse monoclonal antibody,clone 9H8	100 ul	$325 *	Next Day
TA500080	HEK293T cell transient overexpression lysate (LY401675) as a positive control.	20 ug	Included for FREE

WB(1)

IHC(1)

IF(2)

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Also for AKT3 (NM_005465)

Gene Name	Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 1
Synonyms:	DKFZp434N0250; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Immunogen	Recombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 9H8	Isotype	IgG3
Species Reactivity	Human, Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	Western blot: 1:500, IHC 1;50, IF (1:100)
Background	AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1)
Related Pathway	Focal Adhesion Hemostasis Jak-STAT signaling pathway MAPK signaling pathway Signaling by GPCR Toll-like receptor signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3(RC221051) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (TA500080) mouse monoclonal antibody.

IF Image

Anti-AKT3 mouse monoclonal antibody (TA500080) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3(RC221051)

IF Image

Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (TA500080).