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Home Antibody All anti-ACTN1 antibodies

Anti-ACTN1 TRUEMAB Antibody Clone OTI7A4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500072 alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) 100ul
30ul
$379 In Stock
LC400447 ACTN1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500072 Carrier-free (BSA/glycerol-free) alpha-actinin mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(2)
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Also for ACTN1 (NM_001102)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.
Clone NameClone OTI7A4 IsotypeIgG2a
Species ReactivityHuman Concentration0.88 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 102.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens actinin, alpha 1 (ACTN1), transcript variant 2
Alternative NameBDPLT15
Database LinkNP_001093
Entrez Gene 87 Human
FunctionAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Related PathwayES Cell Differentiation/IPSDruggable Genome Focal adhesionAdherens junctionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonSystemic lupus erythematosusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (RC204924, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1.
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Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072)
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072)
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Anti-ACTN1 mouse monoclonal antibody (TA500072) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY ACTN1(RC204924).
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Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody (TA500072).
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