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Home Antibody All anti-SHH antibodies

Anti-SHH TRUEMAB Antibody Clone OTI10H6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500041 SHH (Sonic Hedgehog) mouse monoclonal antibody, clone OTI10H6 (formerly 10H6) 100ul $379 In Stock
LC424868 SHH HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500041 Carrier-free (BSA/glycerol-free) SHH mouse monoclonal antibody, clone OTI10H6 (formerly 10H6) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(1)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 24-197 of human SHH (NP_000184) produced in E.coli.
Clone NameClone OTI10H6 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:1000~2000, IF 1:100,
Predicted MW Explanation 27.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens sonic hedgehog (SHH)
Alternative NameHHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Database LinkNP_000184
Entrez Gene 6469 Human
Entrez Gene 29499 Rat
Entrez Gene 716553 Monkey
Entrez Gene 608860 Dog
FunctionSHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (RC222175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHH.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SHH monoclonal antibody.
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Anti-SHH mouse monoclonal antibody (TA500041) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHH(RC222175).
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