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Anti-Nog TRUEMAB Antibody Clone OTI1H8
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.|
|WB, IHC, IF
||WB 1:20000~40000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens noggin (NOG)|
|SYM1; SYNS1; SYNS1A|
Entrez Gene 9241 Human
|Noggin is a secreted polypeptide which binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, it may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. The results of the mouse knockout of the ortholog suggest that noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.|
|Secreted ProteinDruggable Genome TGF-beta signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
Noggin antibody (1H8) at 1:40000 + Recombinant human Noggin (Cat# RC205020)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500029)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500029)
Anti-Nog mouse monoclonal antibody (TA500029) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).