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Home All anti-Nog antibodies
Anti-Nog TrueMAB Antibody clone 4C12
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
OriGene Data
| Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli. |
| clone 4C12 |
IgG2b |
| Human , Dog |
0.5~1.0 mg/ml (Lot Dependent) |
| WB, IHC, IF |
WB 1:1000~2000, IHC 1:50, IF 1:100, |
| PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose) |
Reference Data
| Homo sapiens noggin (NOG) |
| SYM1; SYNS1 |
| NP_005441 |
| Noggin is a secreted polypeptide which binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, it may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. The results of the mouse knockout of the ortholog suggest that noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. |
TGF Beta Signaling Pathway |
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* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
WB Image
Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin (Cat# RC205020)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-Nog mouse monoclonal antibody. (TA500028)
IF Image
Anti-Nog mouse monoclonal antibody (TA500028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).
IF Image
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody (TA500028).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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