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Anti-HHex TRUEMAB Antibody Clone OTI2H7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for HHex (NM_002729)
|Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex|
||WB: 1:50 - 1:100, IF 1:100
|29.8 kDa(experimental 44KDa)
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-Entry-HHex (RC204815, Right lane) cDNA for 48 hrs and lysed at 1:100 dilution. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody at 1:50 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Hex antibody ( 2H7) at 1:100 dilution + Lysates from HEK-293T cells transfected with human Hex expression vector (Cat# RC204815)
Anti-HHex mouse monoclonal antibody (TA500027) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY HHex(RC204815)