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Anti-IL6 Antibody Polyclonal
|E.coli derived Recombinant Human IL-6 |
||Lot dependent; please refer to CoA along with shipment
|WB, ELISA, IHC
|A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized Human IL-6 matrix.
|Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (1.0 ng/ml), a concentration of 0.20 - 0.40 <=ug/ml of this antibody is required.
|Homo sapiens interleukin 6 (IL6)|
|BSF2; HGF; HSF; IFNB2; IL-6|
|Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
Jak-STAT signaling pathway
Senescence and Autophagy
Toll-like receptor signaling pathway
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To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-6 (60-006BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 0.25 ug/ml-0.5 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information.