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Home Antibody All anti-HIF1A antibodies

Anti-HIF1A Antibody ESEE122

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA326476 Mouse Monoclonal Anti-HIF1 alpha Antibody 100µg $325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to amino acids 329-530
Clone NameESEE122 IsotypeIgG1
Species ReactivityHuman, Mouse, Rat, Cow Concentration1mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsIHC: 1:1000-1:8000, IF/ICC: 8-21ug/ml
BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Purification Protein G Purified
Note Specific for HIF1Alpha

Reference Data

Target Namehypoxia inducible factor 1 alpha subunit
Alternative NameHIF-1-alpha|HIF-1A|HIF-1alpha|HIF1|HIF1-ALPHA|MOP1|PASD8|bHLHe78
Database LinkNP_001521
Entrez Gene 3091 Human
Entrez Gene 15251 Mouse
Entrez Gene 29560 Rat
FunctionHypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia . The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1 is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1 is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1 degradation and lead to its stabilization. In addition, HIF-1 can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway .
Related PathwayTranscription Factors mTOR signaling pathwayPathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody
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IHC Image
IHC analysis of HIF1Alpha tested on mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
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