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Anti-HIF1A Antibody ESEE122
Also for HIF1A (NM_001530)
|Recombinant fragment corresponding to amino acids 329-530|
|Human, Mouse, Rat, Cow
||IHC: 1:1000-1:8000, IF/ICC: 8-21ug/ml
|PBS pH7.4, 50% glycerol, 0.09% sodium azide |
|Protein G Purified
|Specific for HIF1Alpha
|Homo sapiens hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1|
|bHLHe78; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8|
|Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia . The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1 is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1 is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1 degradation and lead to its stabilization. In addition, HIF-1 can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway .|
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Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody
IHC analysis of HIF1Alpha tested on mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.