OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HIF1A antibodies

Anti-HIF1A Antibody ESEE122

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA326476
  • Mouse Monoclonal Anti-HIF1 alpha Antibody
100µg 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to amino acids 329-530
Clone NameESEE122 IsotypeIgG1
Species ReactivityHuman, Mouse, Rat, Cow Concentration1mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsIHC: 1:1000-1:8000, IF/ICC: 8-21ug/ml
BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Purification Protein G Purified
Note Specific for HIF1Alpha

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1
Alternative NamebHLHe78; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Database LinkNP_001521
Entrez Gene 3091 Human
Entrez Gene 15251 Mouse
Entrez Gene 29560 Rat
FunctionHypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia . The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1 is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1 is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1 degradation and lead to its stabilization. In addition, HIF-1 can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway .
Related Pathway
Adipogenesis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody
IHC Image
IHC analysis of HIF1Alpha tested on mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title