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Anti-HSF1 Antibody 10H8
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Also for HSF1 (NM_005526)
|Purified recombinant HSF1 of mouse origin, epitope mapping to amino acids 378-395 |
|Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
||1ug/ml was sufficient for detection of HSF1 by ECL immunoblot in 20ug of HeLa lysate.
|PBS pH7.4, 50% glycerol, 0.09% sodium azide |
|Protein G Purified
|Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots.
|Homo sapiens heat shock transcription factor 1 (HSF1)|
Entrez Gene 3297 Human
Entrez Gene 15499 Mouse
Entrez Gene 79245 Rat
Entrez Gene 714078 Monkey
|HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function . The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) . Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization . There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form . Various experiments have suggested that HFS2 may have roles in differentiation and development .|
|Transcription Factors |
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HSF1 granules present in heat-shocked mitotic cells (green) detected by IF in mitotic heat shocked HeLa cells. Courtesy of Morimoto Lab, Northwestern University, USA.