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Home Antibody All anti-BRAF antibodies

Anti-BRAF PHOSPHO Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA326162 Rabbit polyclonal B-RAF (Phospho-Ser446) antibody 100ul $325 In Stock
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WB(2)
IHC(1)

OriGene Data

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).
Clone Name IsotypeIgG
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%) Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:10000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reference Data

Target NameHomo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)
Alternative NameB-raf; B-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Entrez Gene 673 Human
Entrez Gene 109880 Mouse
Function
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using B-RAF (Phospho-Ser446) antibody.The lane on the right is treated with the synthesized peptide.
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WB Image
Western blot analysis of extracts from K562 cells (Lane 2), JK cells (Lane 3) and 293 cells (Lane 4), using B-RAF (Phospho-Ser446) Antibody. The lane on the left is treated with synthesized peptide.
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IHC Image
Immunohistochemistry analysis of paraffin-embedded human brain tissue using B-RAF (Phospho-Ser446) antibody.The picture on the right is treated with the synthesized peptide.
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