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Anti-VCL PHOSPHO Antibody
|The antiserum was produced against A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyrosine 821|
|Human, Mouse, Rat
||WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
|Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
|Homo sapiens vinculin (VCL), transcript variant 2|
|CMD1W; CMH15; MV; MVCL|
|Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and a-actinin. In addition, vinculin, Talin and a-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression.|
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Western blot analysis of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.