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Anti-VCL PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA325968 Rabbit Polyclonal Vinculin (Tyr821) Antibody (Phospho-specific) 100ul $325 In Stock
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OriGene Data

ImmunogenThe antiserum was produced against A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyrosine 821
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1 mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
Predicted MW Explanation 123kDa
BufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.

Reference Data

Target NameHomo sapiens vinculin (VCL), transcript variant 2
Alternative NameCMD1W; CMH15; HEL114; MV; MVCL
Database LinkNP_003364
Entrez Gene 7414 Human
Entrez Gene 22330 Mouse
Entrez Gene 305679 Rat
FunctionVinculin binding sites have been identified on other cytoskeletal proteins, including Talin and a-actinin. In addition, vinculin, Talin and a-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression.
Related PathwayDruggable Genome Focal adhesionAdherens junctionLeukocyte transendothelial migrationRegulation of actin cytoskeleton

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WB Image
Western blot analysis of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.


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