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Anti-CHEK1 PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA325372 Rabbit Polyclonal Chk1 (Ser280) Antibody (Phospho-specific) 100ul 325
3 Weeks
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OriGene Data

ImmunogenThe antiserum was produced against a synthesized A synthesized peptide derived from human Chk1 around the phosphorylation site of Sersine 280.
Clone Name IsotypeIgG
Species ReactivityHuman, Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1:2000
BufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 2
Alternative NameCHK1
Database LinkNP_001107593
Entrez Gene 1111 Human
FunctionSerine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A.
Related PathwayProtein KinaseStem cell - PluripotencyDruggable Genome Cell cyclep53 signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis on HeLa cell lysate using Phospho-Chk1(Ser280) Antibody


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