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Anti-HDAC2 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA325112
  • Rabbit polyclonal HDAC2 Antibody (Center)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419878) , 20ug Explanation
400ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenThis HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the Central region of human HDAC2.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Chicken) Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:50~100, FC: 1:10~50, IF: 1:10~50
Predicted MW Explanation 55364 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens histone deacetylase 2 (HDAC2), transcript variant 1
Alternative NameHD2; RPD3; YAF1
Database LinkNP_001518
Entrez Gene 3066 Human
Entrez Gene 15182 Mouse
Function
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Cell cycleNotch signaling pathwayHuntington's diseasePathways in cancerChronic myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HDAC2 Antibody (Center) (Cat. #TA325112) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow).
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HDAC2 Antibody (Center) (Cat. #TA325112) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (Center)(Cat#TA325112).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37â). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37â).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37â). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC2 immunoreactivity is localized to Nucleus significantly.
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HDAC2 Antibody (Center) (Cat. #TA325112) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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