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Home Antibody All anti-HMGA1 antibodies

Anti-HMGA1 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA325094 Rabbit polyclonal HMGA1 Antibody (C-term) 400ul $325 3-7 Days
LC419517 HMGA1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IF(1)
FC(1)
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Also for HMGA1 (NM_002131)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat, Hamster) Concentration2.0 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsIF: 1:10~50, WB: 1:1000, FC: 1:10~50
Predicted MW Explanation 11676 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2
Alternative NameHMG-R; HMGA1A; HMGIY
Database LinkNP_002122
Entrez Gene 3159 Human
Entrez Gene 15361 Mouse
Entrez Gene 117062 Rat
Function
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell - PluripotencyTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HMGA1 Antibody (C-term) (Cat. #TA325094) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).
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IF image of A549 cell stained with HMGA1 Antibody (C-term)(Cat#TA325094).A549 cells were incubated with HMGA1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).HMGA1 immunoreactivity is localized to Nucleus significantly.
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HMGA1 Antibody (C-term) (Cat. #TA325094) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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