Home Antibody All anti-HMGA1 antibodies
Also for HMGA1 (NM_002131)
|This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.|
|WB, IF, FC
||WB: 1:1000; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Homo sapiens high mobility group AT-hook 1 (HMGA1), transcript variant 2|
|HMG-R; HMGA1A; HMGIY|
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HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).
Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.).HMGA1 immunoreactivity is localized to Nucleus significantly.
HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.