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Home Antibody All anti-HLA-B antibodies

Anti-HLA-B Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA325053 Rabbit polyclonal HLA-B Antibody (N-term) 400ul $325 3-7 Days
LC432155 MGMT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(5)
IHC(1)
FC(1)
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Also for HLA-B (NM_005514)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000, IHC: 1:10~50, FC: 1:10~50
Predicted MW Explanation 40428 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens major histocompatibility complex, class I, B (HLA-B)
Alternative NameAS; HLAB; SPDA1
Database LinkNP_005505
Entrez Gene 4255 Human
Function
Related PathwayTransmembrane EndocytosisCell adhesion molecules (CAMs)Antigen processing and presentationNatural killer cell mediated cytotoxicityType I diabetes mellitusAutoimmune thyroid diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot analysis of HLA-B Antibody (N-term) (Cat. #TA325053) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
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WB Image
Western blot analysis of HLA-B Antibody (N-term) (Cat. #TA325053) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
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WB Image
Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (Cat. #TA325053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.
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WB Image
Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat. #TA325053). TA325053 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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WB Image
Western blot analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat. #TA325053). TA325053 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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IHC Image
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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FC Image
HLA-B Antibody (N-term) (Cat.#TA325053) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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