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Home Antibody All anti-BRAF antibodies

Anti-BRAF Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324991
  • Rabbit polyclonal B-RAF Antibody (Center)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401382) , 20ug
400ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens v-raf murine sarcoma viral oncogene homolog B (BRAF)
Alternative NameB-RAF1; BRAF1; NS7; RAFB1
Database LinkNP_004324
Function
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of BRAF Antibody (Center) polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human prostata carcinoma reacted with BRAF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Fluorescent image of C2C12 cell stained with BRAF Antibody (Center).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.).BRAF immunoreactivity is localized to Cytoplasm significantly.
FC Image
B-RAF Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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