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Home Antibody All anti-MAPK3 antibodies

Anti-MAPK3 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324941
  • Rabbit polyclonal MAPK3 Antibody (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC421885) , 20ug Explanation
400ul 325 3-7 Days
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WB(1)
IHC(2)
FC(1)
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Also for MAPK3 (NM_001040056)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis MAPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAPK3.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat, Monkey Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000, IHC: 1:50~100, FC: 1:10~50
Predicted MW Explanation 43136 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 2
Alternative NameERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Database LinkNP_001035145
Entrez Gene 5595 Human
Entrez Gene 26417 Mouse
Entrez Gene 50689 Rat
Entrez Gene 708938 Monkey
Function
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab (Cat. #TA324941).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IHC Image
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK1 antibody (N-term) (Cat.#TA324941), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
FC Image
MAPK3 Antibody (N-term) (Cat. #TA324941) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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