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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324933
  • Rabbit polyclonal SMAD2 Antibody (T220)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400372) , 20ug Explanation
400ul $325 3-7 Days

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IF(1)
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Also for SMAD2 (NM_001003652)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from human SMAD2.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat, Zebrafish, Bovine) Concentration0.5 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsIF: 1:200, WB: 1:1000
Predicted MW Explanation 52306 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of SMAD2 Antibody (T220) (Cat.# TA324933) in NCI-H460 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.
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Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324933 SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
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