Home Antibody All anti-SMAD2 antibodies
Also for SMAD2 (NM_001003652)
|This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2.|
|Human, Mouse (Predicted: Rat, Zebrafish, Bovine, Chicken, Drosophila, Pig)
|WB, IF, FC
||IF: 1:100, WB: 1:1000, FC: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
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Western blot analysis of SMAD2 Antibody (Cat.# TA324932) in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324932 SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 Î¼g/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
SMAD2 Antibody (Cat. #TA324932) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.