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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324932
  • Rabbit polyclonal SMAD2 Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400372) , 20ug
400ul 325 3-7 Days
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WB(1)
IF(1)
FC(1)
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Also for SMAD2 (NM_001003652)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1000; IF: 1:100; FC: 1:10-50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 2
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_001003652
Function
Related Pathway
Adherens junction
Cell cycle
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of SMAD2 Antibody in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.
IF Image
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324932 SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
FC Image
SMAD2 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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