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Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).
Western blot analysis of extracts from A431 cells,untreated or treated with EGF using Phospho-EGFR(Tyr1172)(left) or EGFR antibody(right).
Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho EGFR-Y1172 (left) or ErBB2 antibody (right)
Dot blot analysis of anti-EGFR-pY1172 Phospho-specific Pab (Cat.#TA324780) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.