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Home Antibody All anti-STAT3 antibodies

Anti-STAT3 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324777
  • Rabbit polyclonal Phospho-STAT3(S727) Antibody
400ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)

OriGene Data

ImmunogenThis STAT3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:200, WB: 1:1000, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 2
Alternative NameAPRF; HIES
Database LinkNP_003141
Entrez Gene 6774 Human
Entrez Gene 20848 Mouse
Function
Related PathwayTranscription FactorsDruggable Genome Chemokine signaling pathwayJak-STAT signaling pathwayAdipocytokine signaling pathwayPathways in cancerPancreatic cancerAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
The Phospho-STAT3-S727 Pab (Cat. #3260a) is used in western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
WB Image
The phospho-STAT3-Ser727 Pab (Cat. #TA324777) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF Image
Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324777 phospho-STAT3-S727 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).

 

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