Home Antibody All anti-UBE3A antibodies
Also for UBE3A (NM_000462)
|This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-197 amino acids from the N-terminal region of human UBE3A.|
|WB, IHC, IF, FC
||WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens ubiquitin protein ligase E3A (UBE3A), transcript variant 2|
|ANCR; AS; E6-AP; EPVE6AP; HPVE6A|
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
The anti-UBE3A Pab is used in Western blot to detect UBE3A in HeLa cell lysate.
UBE3A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE3A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with UBE3A Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBE3A primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).UBE3A immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
UBE3A Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.