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Home Antibody All anti-UVRAG antibodies

Anti-UVRAG Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324649 Rabbit polyclonal UVRAG Antibody (C-term) 400ul $325 3-7 Days
LC418725 UVRAG HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(2)

OriGene Data

ImmunogenThis UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-699 amino acids from the C-terminal region of human UVRAG.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:10~50, WB: 1:1000, IHC: 1:50~100
Predicted MW Explanation 78151 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens UV radiation resistance associated (UVRAG)
Alternative NameDHTX; p63; VPS38
Database LinkNP_003360
Entrez Gene 7405 Human
Entrez Gene 78610 Mouse
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Immunoprecipitation and western blot analysis of anti-UVRAG Pab (Cat.#TA324649) in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Immunofluorescence staining of Autophagy UVRAG antibody (Cat# TA324649) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
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Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324649 UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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