Home Antibody All anti-MAP1LC3B antibodies
Also for MAP1LC3B (NM_022818)
|This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.|
|WB, IHC, IF
||WB: 1:1000; IHC: 1:50-100; IF: 1:100
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)|
|ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a|
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Western blot analysis of anti-LC3 (APG8b) Pab in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
Western blot analysis of anti-LC3 (APG8b) Pab in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.
Wild-type (Cln3+/+) or homozygous Cln3Äex7/8 (Cln3Äex7/8/Äex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3Äex7/8 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer. Data courtesy of Dr. Susan Cotman, Massachusets General Hospital.
Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 .M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.