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Home Antibody All anti-MAP1LC3A antibodies

Anti-MAP1LC3A Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324624 Rabbit polyclonal LC3 Antibody (APG8A) (N-term) 400ul $325 3-7 Days
LC403169 MAP1LC3A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
IF(1)
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Also for MAP1LC3A (NM_032514)
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OriGene Data

ImmunogenThis LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000, IF: 1:200, IHC: 1:50~100
Predicted MW Explanation 14272 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1
Alternative NameATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Database LinkNP_115903
Entrez Gene 84557 Human
Entrez Gene 66734 Mouse
Entrez Gene 362245 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #TA324624) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
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APG8a (MAP1LC3A) Antibody (M1) (Cat. #TA324624) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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IF image of U251 cells stained with TA324624 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324624 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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