Home Antibody All anti-MAP1LC3A antibodies
Also for MAP1LC3A (NM_032514)
|This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:1000; IHC: 1:50-100; IF: 1:200
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1|
|ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3|
Senescence and Autophagy
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Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
APG8a (MAP1LC3A) Antibody (M1) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).
Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of U251 cells stained with TA324624 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 .M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324624 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.