Home Antibody All anti-HDAC1 antibodies
Also for HDAC1 (NM_004964)
|This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-430 amino acids from the Central region of human HDAC1.|
|Human, Mouse, Rat
||WB: 1:1000, IF: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
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HDAC1 Antibody (S423) (Cat. #TA324609) western blot analysis in Hela cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).
Fluorescent confocal image of Hela cell stained with HDAC1 Antibody (Center S423)(Cat#TA324609).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢). Nuclei were counterstained with DAPI (blue) (10 Âµg/ml, 10 min). HDAC1 immunoreactivity is localized to Nucleus significantly.