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Home Antibody All anti-MAP1LC3A antibodies

Anti-MAP1LC3A Antibody 166AT1234

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324327 Mouse monoclonal LC3 Antibody (APG8) 400ul $325 In Stock
LC403169 MAP1LC3A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
IF(3)
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Also for MAP1LC3A (NM_032514)
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OriGene Data

ImmunogenThis LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Clone Name166AT1234 IsotypeMouse IgG1 k
Species ReactivityHuman, Mouse, Rat Concentration1.09 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:200, WB: 1:1000, IHC: 1:50~100
Predicted MW Explanation 14272 Da
BufferPurified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1
Alternative NameATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Database LinkNP_115903
Entrez Gene 84557 Human
Entrez Gene 66734 Mouse
Entrez Gene 362245 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of anti-LC3 Mab (Cat. #TA324327) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
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Western blot analysis of anti-LC3 Mab (Cat. #TA324327) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
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10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# TA324327. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
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Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8)(Cat. #TA324327). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324327 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8) (Cat. #TA324327). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324327 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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IF image of U251 cells stained with TA324327 LC3 (APG8) antibody.U251 cells were treated with Chloroquine , then incubated with TA324327 LC3 (APG8) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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