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Home Antibody All anti-RPS6KB1 antibodies

Anti-RPS6KB1 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324278
  • Anti-RPS6KB1 (Phospho-Ser424) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for RPS6KB1 (NM_003161)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), transcript variant 1
Alternative Namep70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
Database LinkNP_003152
Entrez Gene 6198 Human
Entrez Gene 72508 Mouse
Entrez Gene 83840 Rat
FunctionPhosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwaymTOR signaling pathwayTGF-beta signaling pathwayFc gamma R-mediated phagocytosisInsulin signaling pathwayAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 70; 85 kDa . Positive control: MCF cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: MCF cells untreated with EGF lysate Lane2: MCF cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
IF Image
Predicted cell location: Cytoplasm, centrosomal and nuclear. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/100. (Original magnification: ×200)

 

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