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Home Antibody All anti-RPS6KB1 antibodies

Anti-RPS6KB1 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324278
  • Anti-RPS6KB1 (Phospho-Ser424) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for RPS6KB1 (NM_003161)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), transcript variant 1
Alternative Namep70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
Database LinkNP_003152
FunctionPhosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Related Pathway
TGF Beta Signaling Pathway

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WB Image
Predicted band size: 70; 85 kDa . Positive control: MCF cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: MCF cells untreated with EGF lysate Lane2: MCF cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>
IF Image
Predicted cell location: Cytoplasm, centrosomal and nuclear. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using RPS6KB1 (Phospho-Ser424) antibody at dilution 1/100. (Original magnification: ×200)</title><style>.apon{position:absolute;clip:rect(471px,auto,auto,471px);}</style><div class=apon>approval <a href=http://crazypaydayloansonline.com >payday loans</a></div>

 

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