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Home Antibody All anti-FOXO4 antibodies

Anti-FOXO4 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324038
  • Anti-FOXO4 (Phospho-Ser197) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for FOXO4 (NM_005938)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human FOXO4.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens forkhead box O4 (FOXO4), transcript variant 1
Alternative NameAFX; AFX1; MLLT7
Database LinkNP_005929
Entrez Gene 4303 Human
Entrez Gene 54601 Mouse
Entrez Gene 302415 Rat
FunctionTranscription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 54 kDa . Positive control: 293 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using FOXO4 (Phospho-Ser197) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using FOXO4 (Phospho-Ser197) antibody at dilution 1/100. (Original magnification:×200)

 

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