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Home Antibody All anti-RELA antibodies

Anti-RELA PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323931
  • Anti-RELA (Phospho-Thr254) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for RELA (NM_021975)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NF?B-p65.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1
Alternative NameNFKB3; p65
Database LinkNP_068810
Entrez Gene 5970 Human
Entrez Gene 19697 Mouse
Entrez Gene 309165 Rat
FunctionNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 65 kDa . Positive control: NIH/3T3 cells untreated or treated with TNF-?× lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cells untreated with TNF-. lysate Lane2: NIH/3T3 cells treated with TNF-. lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using RELA (Phospho-Thr254) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using RELA (Phospho-Thr254) antibody at dilution 1/100. (Original magnification:×200)

 

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