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Home Antibody All anti-PXN antibodies

Anti-PXN PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323769
  • Anti-PXN (Phospho-Tyr118) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
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Also for PXN (NM_025157)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
Predicted MW Explanation 65kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens paxillin (PXN), transcript variant 4
Alternative NameFLJ16691
Database LinkNP_079433
Entrez Gene 5829 Human
Entrez Gene 19303 Mouse
Entrez Gene 360820 Rat
FunctionThis gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties.
Related PathwayStem cell - PluripotencyDruggable Genome Chemokine signaling pathwayVEGF signaling pathwayFocal adhesionLeukocyte transendothelial migrationRegulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 65 kDa . Positive control: NIH/3T3 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cells untreated with serum lysate Lane2: NIH/3T3 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PXN (Phospho-Tyr118) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

 

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