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Home Antibody All anti-LCK antibodies

Anti-LCK PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323287
  • Anti-LCK (phospho-Tyr505) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
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Also for LCK (NM_005356)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 505 (G-Q-Y(p)-Q-P) derived from Human Lck.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1000
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens LCK proto-oncogene, Src family tyrosine kinase (LCK), transcript variant 2
Alternative NameIMD22; LSK; p56lck; pp58lck; YT16
Database LinkNP_005347
FunctionTyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.
Related Pathway
Delta-Notch Signaling Pathway
Hemostasis

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WB Image
Predicted band size: 58 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Treated with the peptide Lane2: Jurkat cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

 

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