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Home Antibody All anti-CRYAB antibodies

Anti-CRYAB Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323096
  • Anti-CRYAB rabbit polyclonal antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419682) , 20ug
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
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Also for CRYAB (NM_001885)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length fusion protein
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:2000-5000, WB: 1:500-2000,IHC: 1:50-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens crystallin, alpha B (CRYAB), transcript variant 1
Alternative NameCMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
Database LinkNP_001876
FunctionCrystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 20kDa. Positive control: Mouse heart and human chromaffin cell tumor tissue, mouse muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Human chromaffin cell tumor tissue lysate Lane3: Mouse muscle tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using CRYAB antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)

 

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