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Anti-PLCG2 PHOSPHO Antibody


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SKU Description Amount Price Availability*  
TA322976 Anti-PLCG2 (Phospho-Tyr753) Rabbit Polyclonal Antibody 100ul 325
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OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLC?2.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500-1000, IF: 1:100-200
Predicted MW Explanation 148kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)
Alternative NameAPLAID; FCAS3; PLC-gamma-2; PLC-IV
Database LinkNP_002652
Entrez Gene 5336 Human
Entrez Gene 234779 Mouse
Entrez Gene 29337 Rat
FunctionThe production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Related PathwayDruggable Genome Inositol phosphate metabolismMetabolic pathwaysErbB signaling pathwayCalcium signaling pathwayPhosphatidylinositol signaling systemVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 148 kDa . Positive control: HepG2 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HepG2 cells untreated with EGF lysate Lane2: HepG2 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

IF Image
Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PLCG2 (Phospho-Tyr753) antibody at dilution 1/100. (Original magnification:×200)


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