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Home Antibody All anti-BAD antibodies

Anti-BAD PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322769
  • Anti-Bad (Phospho-Ser112) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
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Also for BAD (NM_004322)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse Bad.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
Predicted MW Explanation 22kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens BCL2-associated agonist of cell death (BAD), transcript variant 1
Alternative NameBBC2; BCL2L8
Database LinkNP_004313
Entrez Gene 572 Human
Entrez Gene 12015 Mouse
Entrez Gene 64639 Rat
FunctionThe protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Related PathwayDruggable Genome ErbB signaling pathwayApoptosisVEGF signaling pathwayFocal adhesionNeurotrophin signaling pathwayInsulin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 22 kDa . Positive control: Cos7 cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Untreated Cos7 cells lysate Lane2: Cos7 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using Bad (Phospho-Ser112) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

 

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