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Home Antibody All anti-SRC antibodies

Anti-SRC PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322608
  • Anti-SRC (Phospho-Tyr529) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for SRC (NM_005417)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens SRC proto-oncogene, non-receptor tyrosine kinase (SRC), transcript variant 1
Alternative NameASV; c-SRC; p60-Src; SRC1
Database LinkNP_005408
Entrez Gene 6714 Human
Entrez Gene 20779 Mouse
Entrez Gene 83805 Rat
Functionproto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome ErbB signaling pathwayEndocytosisVEGF signaling pathwayFocal adhesionAdherens junctionTight junctionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 60 kDa . Positive control: HT29 cells untreated or treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HT29 cells untreated with PMA lysate Lane2: HT29 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cell membrane; Nucleus; Cytoplasm . Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using SRC (Phospho-Tyr529) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cell membrane; Nucleus; Cytoplasm . Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using SRC (Phospho-Tyr529) antibody at dilution 1/100. (Original magnification:×200)

 

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