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Home Antibody All anti-EIF2S1 antibodies

Anti-EIF2S1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321618
  • Anti-EIF2S1 Rabbit Polyclonal Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401321) , 20ug
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for EIF2S1 (NM_004094)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2a.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)
Alternative NameEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Database LinkNP_004085
FunctionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 36 kDa . Positive control: 3T3, Hela and C6 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 3T3 cells lysate Lane2: Hela cells lysate Lane3: C6 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasmic granule. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using EIF2S1 (Ab-51) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
IF Image
Predicted cell location: Cytoplasmic granule. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using EIF2S1 antibody at dilution 1/100. (Original magnification: ×200)

 

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