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Home Antibody All anti-HSPB1 antibodies

Anti-HSPB1 PHOSPHO Antibody

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SKU Description Amount Price Availability*  
TA321367 Anti-HSPB1 (Phospho-Ser78) Rabbit Polyclonal Antibody 100ul $325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for HSPB1 (NM_001540)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
Predicted MW Explanation 23kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens heat shock 27kDa protein 1 (HSPB1)
Alternative NameCMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Database LinkNP_001531
Entrez Gene 3315 Human
FunctionThe protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Related Pathway MAPK signaling pathwayVEGF signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Predicted band size: 23 kDa . Positive control: HL60 cells treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HL60 cells untreated with UV lysate Lane2: HL60 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
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Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser78) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
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Predicted cell location: Cytoplasmic, Nuclear and centrosomal. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using HSPB1 (Phospho-Ser78) antibody at dilution 1/100. (Original magnification:×200)
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