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Home Antibody All anti-CHUK antibodies

Anti-CHUK PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321322
  • Anti-CHUK (Phospho-Thr23) Rabbit Polyclonal Antibody
100ul $325 2-3 Weeks
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WB(1)
IHC(1)

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)
Alternative NameIKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
Database LinkNP_001269
FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
Related Pathway
Apoptosis
MAPK signaling pathway
Toll-like receptor signaling pathway

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WB Image
Predicted band size: 85 kDa . Positive control: 293 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CHUK (Phospho-Thr23) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

 

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