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Home Antibody All anti-CHEK1 antibodies

Anti-CHEK1 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321319
  • Anti-CHEK1 (Phospho-Ser280) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from Human Chk1.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1000
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3
Alternative NameCHK1
Database LinkNP_001265
Entrez Gene 1111 Human
FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
Related Pathway
Cell cycle

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WB Image
Predicted band size: 54 kDa . Positive control: HepG2 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HepG2 cells untreated with UV lysate Lane2: HepG2 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

 

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